Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1779633..1780223 Replicon chromosome
Accession NZ_CP027064
Organism Klebsiella variicola strain WCHKV030666

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag CLQ71_RS09960 Protein ID WP_008804165.1
Coordinates 1779891..1780223 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag CLQ71_RS09955 Protein ID WP_012541132.1
Coordinates 1779633..1779890 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ71_RS09930 1775242..1775817 + 576 WP_101837112.1 hypothetical protein -
CLQ71_RS09935 1775995..1776831 + 837 WP_008804155.1 alpha/beta hydrolase -
CLQ71_RS09940 1777037..1778008 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
CLQ71_RS09945 1778005..1779105 - 1101 WP_101837113.1 phosphotransferase -
CLQ71_RS09955 1779633..1779890 + 258 WP_012541132.1 antitoxin Antitoxin
CLQ71_RS09960 1779891..1780223 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CLQ71_RS09970 1780481..1781917 - 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
CLQ71_RS09980 1782355..1783809 - 1455 WP_008804170.1 AMP nucleosidase -
CLQ71_RS09985 1783940..1784185 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T97202 WP_008804165.1 NZ_CP027064:1779891-1780223 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T97202 NZ_CP027064:1779891-1780223 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT97202 WP_012541132.1 NZ_CP027064:1779633-1779890 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT97202 NZ_CP027064:1779633-1779890 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References