Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 226259..227169 Replicon plasmid pVir_030666
Accession NZ_CP027063
Organism Klebsiella variicola strain WCHKV030666

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag CLQ71_RS01190 Protein ID WP_004144067.1
Coordinates 226259..226729 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag CLQ71_RS01195 Protein ID WP_004026357.1
Coordinates 226726..227169 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ71_RS01160 222598..222744 - 147 WP_159193595.1 IS3 family transposase -
CLQ71_RS01165 222766..223886 + 1121 WP_101837575.1 IS3 family transposase -
CLQ71_RS01170 223913..224728 - 816 WP_077268817.1 IS3 family transposase -
CLQ71_RS01175 224683..225192 - 510 WP_004901294.1 IS3 family transposase -
CLQ71_RS01185 225888..226148 + 261 WP_048291871.1 hypothetical protein -
CLQ71_RS01190 226259..226729 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
CLQ71_RS01195 226726..227169 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
CLQ71_RS01200 227270..227776 - 507 Protein_226 DUF4113 domain-containing protein -
CLQ71_RS01205 227877..228098 - 222 WP_072060588.1 DUF2188 domain-containing protein -
CLQ71_RS01210 228522..229259 + 738 Protein_228 DUF1311 domain-containing protein -
CLQ71_RS01215 229259..229600 + 342 WP_048291869.1 hypothetical protein -
CLQ71_RS01225 229995..231040 + 1046 Protein_230 IS3 family transposase -
CLQ71_RS28495 231095..231469 + 375 WP_173675457.1 hypothetical protein -
CLQ71_RS28500 231399..231623 + 225 WP_127807699.1 DUF4113 domain-containing protein -
CLQ71_RS01235 231702..231930 + 229 Protein_233 type II toxin-antitoxin system Phd/YefM family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..236161 236161
- flank IS/Tn - - 230342..231040 698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T97198 WP_004144067.1 NZ_CP027063:c226729-226259 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T97198 NZ_CP027063:c226729-226259 [Klebsiella variicola]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTAATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT97198 WP_004026357.1 NZ_CP027063:c227169-226726 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT97198 NZ_CP027063:c227169-226726 [Klebsiella variicola]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTCCTGGGGCAAATCAATGAAGGCCTGGACGGGAAGGTGGCAAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCTTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References