Detailed information of TA system TA097197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 219868..220607
• Replicon: plasmid pVir_030666
• Accession: NZ_CP027063
• Organism: Klebsiella variicola strain WCHKV030666

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CLQ71_RS01140
• Protein ID: WP_021312536.1
• Coordinates: 219868..220353 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: CLQ71_RS01145
• Protein ID: WP_003026799.1
• Coordinates: 220341..220607 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ71_RS01110	215012..215449	-	438	WP_126034224.1	hypothetical protein	-
CLQ71_RS01115	215545..216612	-	1068	WP_048291875.1	hypothetical protein	-
CLQ71_RS01120	216681..216977	-	297	WP_048291874.1	hypothetical protein	-
CLQ71_RS01125	217211..217588	+	378	WP_032719541.1	hypothetical protein	-
CLQ71_RS28490	217785..218759	-	975	WP_048291873.1	hypothetical protein	-
CLQ71_RS01135	219364..219525	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
CLQ71_RS01140	219868..220353	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
CLQ71_RS01145	220341..220607	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
CLQ71_RS01150	220740..221201	-	462	WP_101837567.1	GFA family protein	-
CLQ71_RS01155	221164..222308	-	1145	Protein_218	IS3 family transposase	-
CLQ71_RS01160	222598..222744	-	147	WP_159193595.1	IS3 family transposase	-
CLQ71_RS01165	222766..223886	+	1121	WP_101837575.1	IS3 family transposase	-
CLQ71_RS01170	223913..224728	-	816	WP_077268817.1	IS3 family transposase	-
CLQ71_RS01175	224683..225192	-	510	WP_004901294.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..236161	236161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T97197 WP_021312536.1 NZ_CP027063:c220353-219868 [Klebsiella variicola]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T97197 NZ_CP027063:c220353-219868 [Klebsiella variicola]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT97197 WP_003026799.1 NZ_CP027063:c220607-220341 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT97197 NZ_CP027063:c220607-220341 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66