Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 180108..180633 Replicon plasmid pVir_030666
Accession NZ_CP027063
Organism Klebsiella variicola strain WCHKV030666

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag CLQ71_RS00925 Protein ID WP_004197633.1
Coordinates 180108..180413 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag CLQ71_RS00930 Protein ID WP_015632547.1
Coordinates 180415..180633 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ71_RS00905 176826..177173 + 348 WP_165451970.1 DUF1173 family protein -
CLQ71_RS00910 177322..177627 + 306 WP_126034233.1 hypothetical protein -
CLQ71_RS00915 177949..178734 - 786 WP_048291886.1 site-specific integrase -
CLQ71_RS00920 178933..179921 - 989 Protein_173 hypothetical protein -
CLQ71_RS00925 180108..180413 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
CLQ71_RS00930 180415..180633 - 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CLQ71_RS00940 180912..182285 + 1374 WP_048265005.1 hypothetical protein -
CLQ71_RS00950 182627..183055 - 429 WP_023345594.1 nuclear transport factor 2 family protein -
CLQ71_RS00955 183168..184013 - 846 WP_023345593.1 SDR family oxidoreductase -
CLQ71_RS00960 184116..184511 + 396 WP_023345592.1 helix-turn-helix transcriptional regulator -
CLQ71_RS00965 184623..185207 + 585 WP_023345591.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..236161 236161


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T97195 WP_004197633.1 NZ_CP027063:c180413-180108 [Klebsiella variicola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T97195 NZ_CP027063:c180413-180108 [Klebsiella variicola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAAGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT97195 WP_015632547.1 NZ_CP027063:c180633-180415 [Klebsiella variicola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT97195 NZ_CP027063:c180633-180415 [Klebsiella variicola]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References