Detailed information of TA system TA097192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 60847..61517
• Replicon: plasmid pVir_030666
• Accession: NZ_CP027063
• Organism: Klebsiella variicola strain WCHKV030666

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: CLQ71_RS00355
• Protein ID: WP_004213072.1
• Coordinates: 61074..61517 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: CLQ71_RS00350
• Protein ID: WP_004213073.1
• Coordinates: 60847..61077 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ71_RS00325	57070..57969	-	900	WP_004225022.1	nucleotide-binding protein	-
CLQ71_RS00330	57959..58249	-	291	WP_004213078.1	hypothetical protein	-
CLQ71_RS00335	58601..58807	+	207	WP_004213077.1	hypothetical protein	-
CLQ71_RS00340	58797..59090	-	294	WP_004213076.1	hypothetical protein	-
CLQ71_RS00345	59106..60239	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
CLQ71_RS00350	60847..61077	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CLQ71_RS00355	61074..61517	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
CLQ71_RS00360	61531..61947	-	417	Protein_68	restriction endonuclease	-
CLQ71_RS00365	61951..62244	-	294	Protein_69	transposase	-
CLQ71_RS28475	62661..63296	+	636	Protein_70	response regulator	-
CLQ71_RS00385	63811..64217	-	407	Protein_71	GAF domain-containing protein	-
CLQ71_RS00395	64308..65228	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
CLQ71_RS00400	65277..65768	-	492	WP_011251327.1	DM13 domain-containing protein	-
CLQ71_RS00405	65831..66106	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..236161	236161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T97192 WP_004213072.1 NZ_CP027063:61074-61517 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T97192 NZ_CP027063:61074-61517 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT97192 WP_004213073.1 NZ_CP027063:60847-61077 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT97192 NZ_CP027063:60847-61077 [Klebsiella variicola]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620