Detailed information of TA system TA097191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3416233..3416870
• Replicon: chromosome
• Accession: NZ_CP027061
• Organism: Bacillus sp. ZY-1-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C5I45_RS17115
• Protein ID: WP_003156187.1
• Coordinates: 3416233..3416583 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: C5I45_RS17120
• Protein ID: WP_003156188.1
• Coordinates: 3416589..3416870 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5I45_RS17075	3411273..3411875	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
C5I45_RS17080	3411875..3412663	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
C5I45_RS17085	3412629..3413111	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
C5I45_RS17090	3413108..3413437	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
C5I45_RS17095	3413501..3414508	-	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
C5I45_RS17100	3414520..3414921	-	402	WP_003156178.1	anti-sigma regulatory factor	-
C5I45_RS17105	3414924..3415289	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
C5I45_RS17110	3415294..3416115	-	822	WP_014304404.1	STAS domain-containing protein	-
C5I45_RS17115	3416233..3416583	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C5I45_RS17120	3416589..3416870	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C5I45_RS17125	3416990..3418159	-	1170	WP_025853726.1	alanine racemase	-
C5I45_RS17130	3418276..3419283	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
C5I45_RS17135	3419447..3419812	-	366	WP_014304402.1	holo-ACP synthase	-
C5I45_RS17140	3419905..3420504	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T97191 WP_003156187.1 NZ_CP027061:c3416583-3416233 [Bacillus sp. ZY-1-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T97191 NZ_CP027061:c3416583-3416233 [Bacillus sp. ZY-1-1]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT97191 WP_003156188.1 NZ_CP027061:c3416870-3416589 [Bacillus sp. ZY-1-1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT97191 NZ_CP027061:c3416870-3416589 [Bacillus sp. ZY-1-1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5