Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58381..59117 Replicon plasmid IncFIB IncFII
Accession NZ_CP027054
Organism Klebsiella pneumoniae strain 2_GR_12

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag A6D68_RS27970 Protein ID WP_003026803.1
Coordinates 58381..58863 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A6D68_RS27975 Protein ID WP_003026799.1
Coordinates 58851..59117 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D68_RS27955 54018..54980 + 963 WP_004152113.1 M48 family metalloprotease -
A6D68_RS27960 55068..56322 + 1255 Protein_62 IS3 family transposase -
A6D68_RS27965 56823..58169 - 1347 WP_020314316.1 ISNCY family transposase -
A6D68_RS27970 58381..58863 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
A6D68_RS27975 58851..59117 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A6D68_RS27980 59293..59547 - 255 WP_004152108.1 hypothetical protein -
A6D68_RS27985 59623..59880 - 258 WP_004152107.1 hypothetical protein -
A6D68_RS27990 59929..60132 - 204 WP_004152106.1 hemolysin expression modulator Hha -
A6D68_RS27995 60166..60534 - 369 WP_004152105.1 hypothetical protein -
A6D68_RS28000 60578..61072 - 495 WP_004152104.1 DNA-binding protein -
A6D68_RS28005 61103..61678 - 576 WP_004152103.1 hypothetical protein -
A6D68_RS28010 61666..61935 - 270 WP_004152102.1 hypothetical protein -
A6D68_RS28015 62293..62643 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
A6D68_RS28020 62693..63055 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia - 1..197872 197872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T97187 WP_003026803.1 NZ_CP027054:c58863-58381 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T97187 NZ_CP027054:c58863-58381 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT97187 WP_003026799.1 NZ_CP027054:c59117-58851 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT97187 NZ_CP027054:c59117-58851 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References