Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 37114..37850 Replicon plasmid unnamed
Accession NZ_CP027049
Organism Klebsiella pneumoniae strain 20_GR_12

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag A6D86_RS27325 Protein ID WP_003026803.1
Coordinates 37114..37596 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A6D86_RS27330 Protein ID WP_003026799.1
Coordinates 37584..37850 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D86_RS27310 32751..33713 + 963 WP_004152113.1 M48 family metalloprotease -
A6D86_RS27315 33801..35055 + 1255 Protein_37 IS3 family transposase -
A6D86_RS27320 35556..36902 - 1347 WP_020314316.1 ISNCY family transposase -
A6D86_RS27325 37114..37596 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
A6D86_RS27330 37584..37850 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A6D86_RS27335 38026..38280 - 255 WP_004152108.1 hypothetical protein -
A6D86_RS27340 38356..38613 - 258 WP_004152107.1 hypothetical protein -
A6D86_RS27345 38662..38865 - 204 WP_004152106.1 hemolysin expression modulator Hha -
A6D86_RS27350 38899..39267 - 369 WP_004152105.1 hypothetical protein -
A6D86_RS27355 39311..39805 - 495 WP_004152104.1 DNA-binding protein -
A6D86_RS27360 39836..40411 - 576 WP_004152103.1 hypothetical protein -
A6D86_RS27365 40399..40668 - 270 WP_004152102.1 hypothetical protein -
A6D86_RS27370 41026..41376 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
A6D86_RS27375 41426..41788 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaTEM-1A / blaOXA-9 / aph(3')-Ia - 1..170467 170467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T97168 WP_003026803.1 NZ_CP027049:c37596-37114 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T97168 NZ_CP027049:c37596-37114 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT97168 WP_003026799.1 NZ_CP027049:c37850-37584 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT97168 NZ_CP027049:c37850-37584 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References