Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4118843..4119462 Replicon chromosome
Accession NZ_CP027048
Organism Klebsiella pneumoniae strain 20_GR_12

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag A6D86_RS20800 Protein ID WP_002892050.1
Coordinates 4119244..4119462 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag A6D86_RS20795 Protein ID WP_002892066.1
Coordinates 4118843..4119217 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D86_RS20785 4113995..4115188 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
A6D86_RS20790 4115211..4118357 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
A6D86_RS20795 4118843..4119217 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
A6D86_RS20800 4119244..4119462 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
A6D86_RS20805 4119621..4120187 + 567 WP_002892030.1 maltose O-acetyltransferase -
A6D86_RS29740 4120159..4120299 - 141 WP_004147370.1 hypothetical protein -
A6D86_RS20810 4120320..4120790 + 471 WP_002892026.1 YlaC family protein -
A6D86_RS20815 4120765..4122216 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
A6D86_RS20820 4122317..4123015 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
A6D86_RS20825 4123012..4123152 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
A6D86_RS20830 4123152..4123415 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T97163 WP_002892050.1 NZ_CP027048:4119244-4119462 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T97163 NZ_CP027048:4119244-4119462 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT97163 WP_002892066.1 NZ_CP027048:4118843-4119217 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT97163 NZ_CP027048:4118843-4119217 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References