Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9388..10031 Replicon plasmid IncR IncN
Accession NZ_CP027047
Organism Klebsiella pneumoniae strain 1_GR_13

Toxin (Protein)


Gene name vagD Uniprot ID R9WRW3
Locus tag A6D67_RS28865 Protein ID WP_015063455.1
Coordinates 9615..10031 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag A6D67_RS28860 Protein ID WP_001261276.1
Coordinates 9388..9618 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D67_RS28825 4502..4771 + 270 WP_000339857.1 hypothetical protein -
A6D67_RS28830 4948..5814 - 867 WP_004118283.1 replication initiation protein -
A6D67_RS29875 6349..6453 + 105 WP_032409716.1 hypothetical protein -
A6D67_RS28835 6582..6839 + 258 WP_000764642.1 hypothetical protein -
A6D67_RS28840 6897..7673 - 777 WP_000015958.1 site-specific integrase -
A6D67_RS28845 7670..8413 - 744 WP_000129823.1 hypothetical protein -
A6D67_RS28850 8464..8814 - 351 WP_000493378.1 hypothetical protein -
A6D67_RS28860 9388..9618 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A6D67_RS28865 9615..10031 + 417 WP_015063455.1 type II toxin-antitoxin system VapC family toxin Toxin
A6D67_RS28870 10275..11131 + 857 Protein_13 IS3-like element ISEc15 family transposase -
A6D67_RS28880 11413..12489 + 1077 WP_000227969.1 IS110 family transposase -
A6D67_RS28885 12804..14360 - 1557 WP_053897648.1 PAS domain-containing methyl-accepting chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / blaVIM-27 / aac(6')-Il / dfrA1 / qacE / sul1 / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib - 1..53495 53495


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15036.50 Da        Isoelectric Point: 8.5440

>T97149 WP_015063455.1 NZ_CP027047:9615-10031 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVN

Download         Length: 417 bp

>T97149 NZ_CP027047:9615-10031 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCGTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT97149 WP_001261276.1 NZ_CP027047:9388-9618 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT97149 NZ_CP027047:9388-9618 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9WRW3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References