Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 133865..134601 Replicon plasmid IncFIB IncFII
Accession NZ_CP027044
Organism Klebsiella pneumoniae strain 1_GR_13

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag A6D67_RS27610 Protein ID WP_003026803.1
Coordinates 133865..134347 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A6D67_RS27615 Protein ID WP_003026799.1
Coordinates 134335..134601 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D67_RS27595 130024..131370 - 1347 WP_020314316.1 ISNCY family transposase -
A6D67_RS27600 131600..132232 + 633 WP_001567369.1 hypothetical protein -
A6D67_RS27605 132261..133664 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
A6D67_RS27610 133865..134347 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
A6D67_RS27615 134335..134601 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A6D67_RS27620 134777..135031 - 255 WP_004152108.1 hypothetical protein -
A6D67_RS27625 135107..135364 - 258 WP_004152107.1 hypothetical protein -
A6D67_RS27630 135413..135616 - 204 WP_004152106.1 hemolysin expression modulator Hha -
A6D67_RS27635 135650..135880 - 231 Protein_147 hypothetical protein -
A6D67_RS27645 135934..136637 - 704 Protein_148 IS6-like element IS26 family transposase -
A6D67_RS27650 136817..137557 + 741 WP_001515717.1 tyrosine-type recombinase/integrase -
A6D67_RS27660 138274..139284 - 1011 WP_004152063.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / dfrA14 / aph(3')-Ia / dfrA1 - 1..168873 168873
- inside IScluster/Tn - - 128804..136637 7833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T97148 WP_003026803.1 NZ_CP027044:c134347-133865 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T97148 NZ_CP027044:c134347-133865 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT97148 WP_003026799.1 NZ_CP027044:c134601-134335 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT97148 NZ_CP027044:c134601-134335 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAACCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References