Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1458288..1459024 Replicon chromosome
Accession NZ_CP027042
Organism Klebsiella pneumoniae strain 1_GR_13

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag A6D67_RS07360 Protein ID WP_032433360.1
Coordinates 1458542..1459024 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A6D67_RS07355 Protein ID WP_003026799.1
Coordinates 1458288..1458554 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D67_RS07330 1453934..1455073 + 1140 WP_032433368.1 mannitol dehydrogenase -
A6D67_RS07335 1455102..1455764 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
A6D67_RS07340 1455748..1456752 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
A6D67_RS07345 1456770..1457402 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
A6D67_RS07350 1457412..1457975 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
A6D67_RS07355 1458288..1458554 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A6D67_RS07360 1458542..1459024 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
A6D67_RS07365 1459385..1459984 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
A6D67_RS07370 1460197..1461141 - 945 WP_077254249.1 fimbrial protein -
A6D67_RS07375 1461153..1461731 - 579 WP_032432061.1 type 1 fimbrial protein -
A6D67_RS07380 1461735..1462475 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1425167..1469967 44800


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T97132 WP_032433360.1 NZ_CP027042:1458542-1459024 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T97132 NZ_CP027042:1458542-1459024 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT97132 WP_003026799.1 NZ_CP027042:1458288-1458554 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT97132 NZ_CP027042:1458288-1458554 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References