Detailed information of TA system TA097101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3102827..3103431
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: C1D11_RS15340
• Protein ID: WP_003414492.1
• Coordinates: 3102827..3103219 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: C1D11_RS15345
• Protein ID: WP_003414495.1
• Coordinates: 3103216..3103431 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS15310	3097981..3098769	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
C1D11_RS15315	3099103..3099648	-	546	WP_003904931.1	DUF1802 family protein	-
C1D11_RS15320	3099920..3100804	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C1D11_RS15325	3100807..3101694	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
C1D11_RS15330	3101999..3102540	-	542	Protein_2876	DUF1802 family protein	-
C1D11_RS15335	3102537..3102806	-	270	WP_003414489.1	DUF2277 family protein	-
C1D11_RS15340	3102827..3103219	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
C1D11_RS15345	3103216..3103431	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C1D11_RS15350	3103478..3104227	+	750	WP_003414497.1	enoyl-CoA hydratase	-
C1D11_RS15355	3104306..3105388	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
C1D11_RS15360	3105381..3106691	-	1311	WP_031702645.1	ABC transporter substrate-binding protein	-
C1D11_RS15365	3106694..3107521	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
C1D11_RS15370	3107518..3108429	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T97101 WP_003414492.1 NZ_CP027035:c3103219-3102827 [Mycobacterium tuberculosis variant bovis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T97101 NZ_CP027035:c3103219-3102827 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT97101 WP_003414495.1 NZ_CP027035:c3103431-3103216 [Mycobacterium tuberculosis variant bovis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT97101 NZ_CP027035:c3103431-3103216 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8