Detailed information of TA system TA097099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3076208..3076778
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: C1D11_RS15195
• Protein ID: WP_003414166.1
• Coordinates: 3076208..3076564 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: C1D11_RS15200
• Protein ID: WP_003901465.1
• Coordinates: 3076548..3076778 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS15175	3071660..3073348	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
C1D11_RS15180	3073352..3073678	-	327	WP_003414157.1	hypothetical protein	-
C1D11_RS15185	3073851..3074438	+	588	WP_003914429.1	DUF3558 family protein	-
C1D11_RS15190	3074457..3076106	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
C1D11_RS15195	3076208..3076564	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
C1D11_RS15200	3076548..3076778	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
C1D11_RS15205	3076821..3077864	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
C1D11_RS15210	3078055..3078330	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
C1D11_RS21670	3078506..3078760	-	255	WP_003917684.1	hypothetical protein	-
C1D11_RS15220	3078908..3079312	+	405	WP_003414181.1	hypothetical protein	-
C1D11_RS15225	3079309..3079500	+	192	WP_003414184.1	hypothetical protein	-
C1D11_RS15230	3079564..3080853	+	1290	Protein_2856	transposase family protein	-
C1D11_RS15235	3081087..3081344	+	258	WP_003899489.1	hypothetical protein	-
C1D11_RS15240	3081449..3081760	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T97099 WP_003414166.1 NZ_CP027035:c3076564-3076208 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T97099 NZ_CP027035:c3076564-3076208 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT97099 WP_003901465.1 NZ_CP027035:c3076778-3076548 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT97099 NZ_CP027035:c3076778-3076548 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3