Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3076208..3076778 Replicon chromosome
Accession NZ_CP027035
Organism Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag C1D11_RS15195 Protein ID WP_003414166.1
Coordinates 3076208..3076564 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag C1D11_RS15200 Protein ID WP_003901465.1
Coordinates 3076548..3076778 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1D11_RS15175 3071660..3073348 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
C1D11_RS15180 3073352..3073678 - 327 WP_003414157.1 hypothetical protein -
C1D11_RS15185 3073851..3074438 + 588 WP_003914429.1 DUF3558 family protein -
C1D11_RS15190 3074457..3076106 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
C1D11_RS15195 3076208..3076564 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
C1D11_RS15200 3076548..3076778 - 231 WP_003901465.1 antitoxin MazE Antitoxin
C1D11_RS15205 3076821..3077864 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
C1D11_RS15210 3078055..3078330 + 276 WP_003911993.1 DUF1778 domain-containing protein -
C1D11_RS21670 3078506..3078760 - 255 WP_003917684.1 hypothetical protein -
C1D11_RS15220 3078908..3079312 + 405 WP_003414181.1 hypothetical protein -
C1D11_RS15225 3079309..3079500 + 192 WP_003414184.1 hypothetical protein -
C1D11_RS15230 3079564..3080853 + 1290 Protein_2856 transposase family protein -
C1D11_RS15235 3081087..3081344 + 258 WP_003899489.1 hypothetical protein -
C1D11_RS15240 3081449..3081760 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T97099 WP_003414166.1 NZ_CP027035:c3076564-3076208 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T97099 NZ_CP027035:c3076564-3076208 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT97099 WP_003901465.1 NZ_CP027035:c3076778-3076548 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT97099 NZ_CP027035:c3076778-3076548 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References