Detailed information of TA system TA097097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3036212..3036891
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: C1D11_RS14965
• Protein ID: WP_003414059.1
• Coordinates: 3036212..3036628 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: C1D11_RS14970
• Protein ID: WP_003414061.1
• Coordinates: 3036625..3036891 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS14945	3032264..3033166	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C1D11_RS14950	3033235..3033987	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
C1D11_RS14955	3034231..3034506	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
C1D11_RS14960	3034503..3036125	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
C1D11_RS14965	3036212..3036628	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
C1D11_RS14970	3036625..3036891	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C1D11_RS14975	3036917..3037312	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
C1D11_RS14980	3037309..3037578	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
C1D11_RS14985	3037588..3038682	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
C1D11_RS14990	3038679..3039098	-	420	Protein_2810	winged helix-turn-helix domain-containing protein	-
C1D11_RS15000	3039172..3039651	-	480	WP_003414073.1	dihydrofolate reductase	-
C1D11_RS15005	3039722..3040522	-	801	WP_003911953.1	thymidylate synthase	-
C1D11_RS15010	3040678..3041415	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T97097 WP_003414059.1 NZ_CP027035:c3036628-3036212 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T97097 NZ_CP027035:c3036628-3036212 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT97097 WP_003414061.1 NZ_CP027035:c3036891-3036625 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT97097 NZ_CP027035:c3036891-3036625 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64