Detailed information of TA system TA097086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2246589..2247215
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: C1D11_RS10935
• Protein ID: WP_003410075.1
• Coordinates: 2246817..2247215 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C1D11_RS10930
• Protein ID: WP_019283586.1
• Coordinates: 2246589..2246816 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS10920	2244628..2244972	-	345	WP_003410065.1	ferredoxin family protein	-
C1D11_RS10925	2245161..2246330	-	1170	WP_003899126.1	ATP-binding protein	-
C1D11_RS10930	2246589..2246816	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C1D11_RS10935	2246817..2247215	+	399	WP_003410075.1	PIN domain nuclease	Toxin
C1D11_RS10940	2247398..2247829	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
C1D11_RS10945	2247930..2248364	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
C1D11_RS10960	2248801..2248980	-	180	Protein_2058	hypothetical protein	-
C1D11_RS10965	2249041..2249688	+	648	WP_071854215.1	transposase	-
C1D11_RS10970	2249642..2250232	+	591	WP_003899131.1	IS110 family transposase	-
C1D11_RS10975	2250360..2251616	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T97086 WP_003410075.1 NZ_CP027035:2246817-2247215 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T97086 NZ_CP027035:2246817-2247215 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT97086 WP_019283586.1 NZ_CP027035:2246589-2246816 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT97086 NZ_CP027035:2246589-2246816 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG