Detailed information of TA system TA097080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195907..2196610
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: C1D11_RS10610
• Protein ID: WP_003409778.1
• Coordinates: 2195907..2196236 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: C1D11_RS10615
• Protein ID: WP_003409780.1
• Coordinates: 2196233..2196610 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS10590	2192290..2193360	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
C1D11_RS10595	2193357..2193872	+	516	WP_003409718.1	flavin reductase family protein	-
C1D11_RS10600	2193869..2194930	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
C1D11_RS10605	2194927..2195697	+	771	WP_003409775.1	SDR family oxidoreductase	-
C1D11_RS10610	2195907..2196236	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C1D11_RS10615	2196233..2196610	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
C1D11_RS10620	2196607..2197197	-	591	WP_003409784.1	SEC-C domain-containing protein	-
C1D11_RS10625	2197252..2198616	+	1365	WP_003899094.1	HNH endonuclease	-
C1D11_RS10630	2198771..2199223	-	453	WP_010950636.1	lipoprotein	-
C1D11_RS10635	2199287..2199688	+	402	WP_003409869.1	hypothetical protein	-
C1D11_RS10640	2199681..2199863	-	183	WP_003409870.1	hypothetical protein	-
C1D11_RS10645	2199977..2200327	-	351	WP_003409871.1	hypothetical protein	-
C1D11_RS10650	2200338..2201240	-	903	WP_003409874.1	hypothetical protein	-
C1D11_RS10655	2201261..2201452	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T97080 WP_003409778.1 NZ_CP027035:c2196236-2195907 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T97080 NZ_CP027035:c2196236-2195907 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT97080 WP_003409780.1 NZ_CP027035:c2196610-2196233 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT97080 NZ_CP027035:c2196610-2196233 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8