Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1691450..1692066 Replicon chromosome
Accession NZ_CP027035
Organism Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag C1D11_RS08275 Protein ID WP_003407593.1
Coordinates 1691749..1692066 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag C1D11_RS08270 Protein ID WP_003900349.1
Coordinates 1691450..1691752 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1D11_RS08260 1687336..1689183 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
C1D11_RS08265 1689184..1691436 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
C1D11_RS08270 1691450..1691752 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
C1D11_RS08275 1691749..1692066 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
C1D11_RS08280 1692063..1693067 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
C1D11_RS08285 1693120..1694409 + 1290 WP_003407599.1 serine hydrolase -
C1D11_RS08290 1694482..1695255 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
C1D11_RS08295 1695313..1695504 - 192 WP_003407609.1 dodecin family protein -
C1D11_RS21535 1695535..1695759 - 225 WP_160522526.1 NUDIX domain-containing protein -
C1D11_RS08305 1696029..1697057 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1684502..1699002 14500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T97076 WP_003407593.1 NZ_CP027035:1691749-1692066 [Mycobacterium tuberculosis variant bovis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T97076 NZ_CP027035:1691749-1692066 [Mycobacterium tuberculosis variant bovis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT97076 WP_003900349.1 NZ_CP027035:1691450-1691752 [Mycobacterium tuberculosis variant bovis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT97076 NZ_CP027035:1691450-1691752 [Mycobacterium tuberculosis variant bovis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References