Detailed information of TA system TA097068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1024479..1025452
• Replicon: chromosome
• Accession: NZ_CP027035
• Organism: Mycobacterium tuberculosis variant bovis strain 2002/0476

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: C1D11_RS05045
• Protein ID: WP_003404756.1
• Coordinates: 1024952..1025452 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: C1D11_RS05040
• Protein ID: WP_003404754.1
• Coordinates: 1024479..1024955 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1D11_RS05020	1020326..1021597	-	1272	WP_003404747.1	PPE family protein	-
C1D11_RS05025	1021612..1021911	-	300	WP_003404748.1	PE family protein	-
C1D11_RS05030	1022355..1024136	+	1782	WP_003404749.1	BCCT family transporter	-
C1D11_RS05035	1024133..1024390	+	258	WP_003404750.1	hypothetical protein	-
C1D11_RS05040	1024479..1024955	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
C1D11_RS05045	1024952..1025452	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
C1D11_RS05055	1025765..1027084	-	1320	WP_010950468.1	IS256-like element IS1554 family transposase	-
C1D11_RS05060	1027372..1027953	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
C1D11_RS05065	1027953..1029605	+	1653	WP_003404761.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1024133..1070046	45913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T97068 WP_003404756.1 NZ_CP027035:1024952-1025452 [Mycobacterium tuberculosis variant bovis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T97068 NZ_CP027035:1024952-1025452 [Mycobacterium tuberculosis variant bovis]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT97068 WP_003404754.1 NZ_CP027035:1024479-1024955 [Mycobacterium tuberculosis variant bovis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT97068 NZ_CP027035:1024479-1024955 [Mycobacterium tuberculosis variant bovis]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTGGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2

Antitoxin

• AlphaFold DB: G0TFR4