Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 499160..499796 Replicon chromosome
Accession NZ_CP027034
Organism Bacillus pumilus strain 150a

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag C5Y82_RS02420 Protein ID WP_003214169.1
Coordinates 499446..499796 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag C5Y82_RS02415 Protein ID WP_012008995.1
Coordinates 499160..499441 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5Y82_RS02395 495305..495910 - 606 WP_119124432.1 rhomboid family intramembrane serine protease -
C5Y82_RS02400 496005..496370 + 366 WP_119124433.1 holo-ACP synthase -
C5Y82_RS02405 496531..497547 + 1017 WP_088004450.1 outer membrane lipoprotein carrier protein LolA -
C5Y82_RS02410 497685..498863 + 1179 WP_119124434.1 alanine racemase -
C5Y82_RS02415 499160..499441 + 282 WP_012008995.1 hypothetical protein Antitoxin
C5Y82_RS02420 499446..499796 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C5Y82_RS02425 499911..500741 + 831 WP_119124435.1 RsbT co-antagonist protein RsbRA -
C5Y82_RS02430 500746..501114 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
C5Y82_RS02435 501117..501518 + 402 WP_003214085.1 anti-sigma regulatory factor -
C5Y82_RS02440 501529..502536 + 1008 WP_119124436.1 PP2C family protein-serine/threonine phosphatase -
C5Y82_RS02445 502596..502925 + 330 WP_061406832.1 anti-sigma factor antagonist -
C5Y82_RS02450 502922..503410 + 489 WP_119124437.1 anti-sigma B factor RsbW -
C5Y82_RS02455 503376..504164 + 789 WP_058015397.1 RNA polymerase sigma factor SigB -
C5Y82_RS02460 504164..504763 + 600 WP_119124438.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T97049 WP_003214169.1 NZ_CP027034:499446-499796 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T97049 NZ_CP027034:499446-499796 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGGTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAAATT
CGTACAATCGATAAACAAAGACTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT97049 WP_012008995.1 NZ_CP027034:499160-499441 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT97049 NZ_CP027034:499160-499441 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References