Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 103560..104119 | Replicon | plasmid unnamed |
| Accession | NZ_CP027028 | ||
| Organism | Shigella dysenteriae strain E670/74 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | C5S64_RS27915 | Protein ID | WP_000421262.1 |
| Coordinates | 103560..103835 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | C5S64_RS27920 | Protein ID | WP_001178088.1 |
| Coordinates | 103835..104119 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C5S64_RS28610 | 98815..98910 | - | 96 | Protein_112 | transposase domain-containing protein | - |
| C5S64_RS27885 | 99038..100069 | + | 1032 | WP_005011710.1 | IS630 family transposase | - |
| C5S64_RS27890 | 100101..100406 | - | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| C5S64_RS27895 | 100408..100626 | - | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| C5S64_RS27900 | 101161..102252 | - | 1092 | Protein_116 | IS66 family transposase | - |
| C5S64_RS27905 | 102260..102830 | + | 571 | Protein_117 | plasmid segregation protein ParM | - |
| C5S64_RS27910 | 102833..103184 | + | 352 | Protein_118 | plasmid partitioning/stability family protein | - |
| C5S64_RS29035 | 103291..103428 | + | 138 | WP_000766699.1 | hypothetical protein | - |
| C5S64_RS27915 | 103560..103835 | - | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C5S64_RS27920 | 103835..104119 | - | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C5S64_RS27930 | 104643..104822 | + | 180 | Protein_122 | MFS transporter | - |
| C5S64_RS27935 | 104872..105081 | + | 210 | Protein_123 | peptidoglycan-binding protein | - |
| C5S64_RS27940 | 105181..105366 | - | 186 | Protein_124 | ATP-binding protein | - |
| C5S64_RS27945 | 105570..106244 | + | 675 | WP_004967157.1 | IS66 family insertion sequence hypothetical protein | - |
| C5S64_RS27950 | 106241..106588 | + | 348 | WP_000631725.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C5S64_RS27955 | 106608..108165 | + | 1558 | Protein_127 | IS66 family transposase | - |
| C5S64_RS27960 | 108210..108608 | + | 399 | Protein_128 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 | 1..149179 | 149179 | |
| - | inside | IScluster/Tn | - | ospG / ipaH9.8 / ospI | 93565..116806 | 23241 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T97042 WP_000421262.1 NZ_CP027028:c103835-103560 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T97042 NZ_CP027028:c103835-103560 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT97042 WP_001178088.1 NZ_CP027028:c104119-103835 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT97042 NZ_CP027028:c104119-103835 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q327A8 |