Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 100101..100626 Replicon plasmid unnamed
Accession NZ_CP027028
Organism Shigella dysenteriae strain E670/74

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag C5S64_RS27890 Protein ID WP_001159860.1
Coordinates 100101..100406 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag C5S64_RS27895 Protein ID WP_000813626.1
Coordinates 100408..100626 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5S64_RS27865 95597..96187 - 591 WP_000705601.1 type III secretion system effector protein kinase OspG -
C5S64_RS29145 96216..96442 - 227 Protein_109 type II toxin-antitoxin system RelE/ParE family toxin -
C5S64_RS27875 96439..96708 - 270 WP_000079956.1 hypothetical protein -
C5S64_RS27880 96916..98553 - 1638 WP_105242117.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
C5S64_RS28610 98815..98910 - 96 Protein_112 transposase domain-containing protein -
C5S64_RS27885 99038..100069 + 1032 WP_005011710.1 IS630 family transposase -
C5S64_RS27890 100101..100406 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
C5S64_RS27895 100408..100626 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C5S64_RS27900 101161..102252 - 1092 Protein_116 IS66 family transposase -
C5S64_RS27905 102260..102830 + 571 Protein_117 plasmid segregation protein ParM -
C5S64_RS27910 102833..103184 + 352 Protein_118 plasmid partitioning/stability family protein -
C5S64_RS29035 103291..103428 + 138 WP_000766699.1 hypothetical protein -
C5S64_RS27915 103560..103835 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
C5S64_RS27920 103835..104119 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein -
C5S64_RS27930 104643..104822 + 180 Protein_122 MFS transporter -
C5S64_RS27935 104872..105081 + 210 Protein_123 peptidoglycan-binding protein -
C5S64_RS27940 105181..105366 - 186 Protein_124 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 1..149179 149179
- inside IScluster/Tn - ospG / ipaH9.8 / ospI 93565..116806 23241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T97041 WP_001159860.1 NZ_CP027028:c100406-100101 [Shigella dysenteriae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T97041 NZ_CP027028:c100406-100101 [Shigella dysenteriae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT97041 WP_000813626.1 NZ_CP027028:c100626-100408 [Shigella dysenteriae]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT97041 NZ_CP027028:c100626-100408 [Shigella dysenteriae]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References