Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 61430..62205 | Replicon | plasmid unnamed |
| Accession | NZ_CP027028 | ||
| Organism | Shigella dysenteriae strain E670/74 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | Q326V1 |
| Locus tag | C5S64_RS27650 | Protein ID | WP_000405248.1 |
| Coordinates | 61430..61912 (-) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | - |
| Locus tag | C5S64_RS27655 | Protein ID | WP_075330081.1 |
| Coordinates | 61903..62205 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C5S64_RS27610 | 56821..57207 | - | 387 | Protein_64 | KUP/HAK/KT family potassium transporter | - |
| C5S64_RS27615 | 57320..57508 | + | 189 | WP_000957853.1 | hypothetical protein | - |
| C5S64_RS27620 | 57518..58717 | + | 1200 | WP_105242110.1 | IS91 family transposase | - |
| C5S64_RS27625 | 58901..59161 | - | 261 | Protein_67 | IS3 family transposase | - |
| C5S64_RS27630 | 59235..59573 | + | 339 | Protein_68 | transposase | - |
| C5S64_RS27635 | 59616..59879 | - | 264 | Protein_69 | IS3 family transposase | - |
| C5S64_RS27640 | 59941..61092 | + | 1152 | WP_001254932.1 | IS30-like element IS30 family transposase | - |
| C5S64_RS27645 | 61102..61362 | - | 261 | Protein_71 | transposase | - |
| C5S64_RS27650 | 61430..61912 | - | 483 | WP_000405248.1 | GNAT family N-acetyltransferase | Toxin |
| C5S64_RS27655 | 61903..62205 | - | 303 | WP_075330081.1 | DUF1778 domain-containing protein | Antitoxin |
| C5S64_RS27665 | 62477..62749 | + | 273 | WP_000019158.1 | hypothetical protein | - |
| C5S64_RS27670 | 62730..63853 | + | 1124 | Protein_75 | IS91 family transposase | - |
| C5S64_RS28600 | 65445..65573 | + | 129 | Protein_77 | IS5/IS1182 family transposase | - |
| C5S64_RS27690 | 65722..67176 | - | 1455 | WP_105242111.1 | type 3 secretion system effector OspC2 | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 | 1..149179 | 149179 | |
| - | inside | IScluster/Tn | - | ospC3 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 | 32172..67998 | 35826 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17736.50 Da Isoelectric Point: 7.0382
>T97039 WP_000405248.1 NZ_CP027028:c61912-61430 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
Download Length: 483 bp
>T97039 NZ_CP027028:c61912-61430 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11717.33 Da Isoelectric Point: 4.9167
>AT97039 WP_075330081.1 NZ_CP027028:c62205-61903 [Shigella dysenteriae]
MEVFMSTVASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK
MEVFMSTVASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK
Download Length: 303 bp
>AT97039 NZ_CP027028:c62205-61903 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGTTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA
ATGGAGGTTTTTATGTCTACAGTTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|