Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 43246..44003 Replicon plasmid unnamed
Accession NZ_CP027028
Organism Shigella dysenteriae strain E670/74

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag C5S64_RS27520 Protein ID WP_000501969.1
Coordinates 43518..44003 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C5S64_RS27515 Protein ID WP_011114751.1
Coordinates 43246..43530 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5S64_RS27480 39331..40005 + 675 WP_001088287.1 IS66 family insertion sequence hypothetical protein -
C5S64_RS27485 40002..40349 + 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
C5S64_RS27490 40369..41926 + 1558 Protein_46 IS66 family transposase -
C5S64_RS29020 42180..42344 - 165 WP_032336446.1 hypothetical protein -
C5S64_RS27515 43246..43530 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C5S64_RS27520 43518..44003 + 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
C5S64_RS27525 44176..44508 - 333 Protein_50 IS4/IS5 family transposase -
C5S64_RS27530 44572..44832 + 261 Protein_51 transposase -
C5S64_RS27535 44897..46048 + 1152 WP_001254932.1 IS30-like element IS30 family transposase -
C5S64_RS27540 46055..46480 + 426 Protein_53 IS3 family transposase -
C5S64_RS27545 46497..47015 + 519 Protein_54 transposase -
C5S64_RS28675 47382..48834 + 1453 Protein_55 type 3 secretion system effector OspC4 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 1..149179 149179
- inside IScluster/Tn - ospC3 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 32172..67998 35826


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T97038 WP_000501969.1 NZ_CP027028:43518-44003 [Shigella dysenteriae]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T97038 NZ_CP027028:43518-44003 [Shigella dysenteriae]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT97038 WP_011114751.1 NZ_CP027028:43246-43530 [Shigella dysenteriae]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT97038 NZ_CP027028:43246-43530 [Shigella dysenteriae]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References