Detailed information of TA system TA097024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2664199..2664788
• Replicon: chromosome
• Accession: NZ_CP027027
• Organism: Shigella dysenteriae strain E670/74

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A398TMA1
• Locus tag: C5S64_RS14210
• Protein ID: WP_000513533.1
• Coordinates: 2664199..2664447 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C5S64_RS14215
• Protein ID: WP_001016018.1
• Coordinates: 2664444..2664788 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5S64_RS14170	2659204..2660109	+	906	Protein_2548	IS3 family transposase	-
C5S64_RS14175	2660119..2660463	+	345	Protein_2549	IS30 family transposase	-
C5S64_RS14180	2660470..2661366	+	897	Protein_2550	IS3 family transposase	-
C5S64_RS14185	2661407..2661757	-	351	WP_000459725.1	recombinase RecT	-
C5S64_RS14190	2661775..2662056	-	282	WP_000995347.1	host nuclease inhibitor GamL	-
C5S64_RS14195	2662077..2662358	-	282	WP_000934194.1	hypothetical protein	-
C5S64_RS14200	2662370..2662582	-	213	WP_000917251.1	hypothetical protein	-
C5S64_RS14205	2662653..2663543	-	891	WP_075329961.1	Rha family phage regulatory protein	-
C5S64_RS14210	2664199..2664447	+	249	WP_000513533.1	type II toxin-antitoxin system HicA family toxin	Toxin
C5S64_RS14215	2664444..2664788	+	345	WP_001016018.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C5S64_RS28830	2664903..2665073	-	171	WP_164834428.1	hypothetical protein	-
C5S64_RS14220	2665195..2665914	-	720	WP_000162429.1	LexA family transcriptional regulator	-
C5S64_RS14225	2666020..2666259	+	240	WP_000171144.1	helix-turn-helix transcriptional regulator	-
C5S64_RS28655	2666422..2666607	+	186	WP_005011604.1	hypothetical protein	-
C5S64_RS14235	2666785..2667171	+	387	WP_000470023.1	hypothetical protein	-
C5S64_RS14240	2667165..2668670	+	1506	WP_000912605.1	DEAD/DEAH box helicase	-
C5S64_RS14245	2668667..2669563	-	897	Protein_2564	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH4.5	2635431..2681515	46084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.07 Da
• Isoelectric Point: 10.3195

>T97024 WP_000513533.1 NZ_CP027027:2664199-2664447 [Shigella dysenteriae]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T97024 NZ_CP027027:2664199-2664447 [Shigella dysenteriae]
ATGGGGAAAGCAGATAAGCTACTGGAAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAGTTGGTCGTTTTGTT
TTCCTCTCTGGGATATGTCAAAAAGGAAATGCAGGGCTCAAGAGTGCGGTTTTTCAATGCTGAAATCAACCACACCATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCGATTAAACAGAATCTGAAAGAGGCTGGG
GTATTATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13051.73 Da
• Isoelectric Point: 4.5590

>AT97024 WP_001016018.1 NZ_CP027027:2664444-2664788 [Shigella dysenteriae]
MNHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Nucleotide

• Length: 345 bp

>AT97024 NZ_CP027027:2664444-2664788 [Shigella dysenteriae]
ATGAATCATCTAAAATATAAAGGATATTTAGGTACAGTAGAACCAGATTTTGAAAATAATATCCTGTACGGAAAACTGGC
GTTTATTCGAGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAGACATCGGTTGAGCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAGCCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGACTTGATCCAGAA
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCGCTGGAAAAAGA
AGTCAACCATCACACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A398TMA1