Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1970576..1971194 Replicon chromosome
Accession NZ_CP027027
Organism Shigella dysenteriae strain E670/74

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5S64_RS10380 Protein ID WP_001291435.1
Coordinates 1970976..1971194 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A193LN30
Locus tag C5S64_RS10375 Protein ID WP_000344802.1
Coordinates 1970576..1970950 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5S64_RS10365 1965665..1966858 + 1194 WP_024258956.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C5S64_RS10370 1966881..1970030 + 3150 WP_001132466.1 multidrug efflux RND transporter permease subunit -
C5S64_RS10375 1970576..1970950 + 375 WP_000344802.1 Hha toxicity modulator TomB Antitoxin
C5S64_RS10380 1970976..1971194 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C5S64_RS10385 1971366..1971917 + 552 WP_000102564.1 maltose O-acetyltransferase -
C5S64_RS10390 1972033..1972503 + 471 WP_000136192.1 YlaC family protein -
C5S64_RS10395 1972667..1974217 + 1551 WP_024259118.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5S64_RS10400 1974259..1974612 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C5S64_RS10405 1975017..1975468 - 452 Protein_1873 hypothetical protein -
C5S64_RS10410 1975669..1975896 - 228 WP_000513895.1 DUF2188 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T97023 WP_001291435.1 NZ_CP027027:1970976-1971194 [Shigella dysenteriae]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T97023 NZ_CP027027:1970976-1971194 [Shigella dysenteriae]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14587.42 Da        Isoelectric Point: 4.7395

>AT97023 WP_000344802.1 NZ_CP027027:1970576-1970950 [Shigella dysenteriae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNTTKENPASLSC

Download         Length: 375 bp

>AT97023 NZ_CP027027:1970576-1970950 [Shigella dysenteriae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATACGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A193LN30

References