Detailed information of TA system TA096987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3524724..3525520
• Replicon: chromosome
• Accession: NZ_CP026985
• Organism: Pectobacterium parmentieri strain IFB5619

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: C5E25_RS16385
• Protein ID: WP_121247144.1
• Coordinates: 3524724..3525230 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: C5E25_RS16390
• Protein ID: WP_100683471.1
• Coordinates: 3525227..3525520 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E25_RS23220	3520204..3520374	-	171	WP_012773209.1	hypothetical protein	-
C5E25_RS16365	3520515..3520856	+	342	WP_121247131.1	hypothetical protein	-
C5E25_RS16375	3521767..3522495	+	729	WP_121247138.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
C5E25_RS16380	3522936..3524408	-	1473	WP_121247141.1	hypothetical protein	-
C5E25_RS16385	3524724..3525230	-	507	WP_121247144.1	GNAT family N-acetyltransferase	Toxin
C5E25_RS16390	3525227..3525520	-	294	WP_100683471.1	DUF1778 domain-containing protein	Antitoxin
C5E25_RS16395	3525941..3527200	+	1260	WP_121247147.1	integrase arm-type DNA-binding domain-containing protein	-
C5E25_RS16410	3528104..3528325	-	222	WP_010301503.1	helix-turn-helix domain-containing protein	-
C5E25_RS16415	3528446..3528706	+	261	WP_121247153.1	helix-turn-helix domain-containing protein	-
C5E25_RS16420	3528810..3529076	+	267	WP_121247156.1	helix-turn-helix domain-containing protein	-
C5E25_RS16425	3529467..3529724	+	258	WP_121247159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3518622..3525777	7155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18176.12 Da
• Isoelectric Point: 6.4763

>T96987 WP_121247144.1 NZ_CP026985:c3525230-3524724 [Pectobacterium parmentieri]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAIATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGILVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SILLEVEI

Nucleotide

• Length: 507 bp

>T96987 NZ_CP026985:c3525230-3524724 [Pectobacterium parmentieri]
ATGATTTCCGCCCCTGAGCCACTCCACGCCGAACATGTATTATCTTCATTCTGTTGTGGCGTGGAGTCTATGGATAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGTGCATCACGGACTTTCGTTAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCTATAGCAACGAATGCCGCTCCCGGACGTTTTCGCCGCAATATGCCCGATCCTATT
CCGGTCGTGGTACTGGGTCGTTTGGCAGTAGATAAATCATTGCATGGTCAGGGTGTTGGCCGTGCTTTGGTACGGGATGC
TGGGCTAAGAGTGATTCAGGTGGCGGATACTATCGGCATCCGAGGAATATTGGTCCATGCGCTGTCTGATGAAGCGCGGG
AATTTTACCTACGGGTGGGGTTTGAGCCTTCACCGATTGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTGGGG
AGCATTTTACTTGAAGTCGAAATCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10900.50 Da
• Isoelectric Point: 10.1968

>AT96987 WP_100683471.1 NZ_CP026985:c3525520-3525227 [Pectobacterium parmentieri]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT96987 NZ_CP026985:c3525520-3525227 [Pectobacterium parmentieri]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTGCGCATCAAGCCCGCCGAACGCGATCTGATTGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTCCTTGAAGCTGCTCGCGCCGCCGCAGAGGAAGCGTTGA
TCGATCAACGTATCATCATGGCAGATCCTGATGCTTATCAGGAATTCCTTGCCCGTTTGGATCAGGCTCCTTCGCCGAAT
GCGGCGCTACGCAAAACCATGCAAACGCCCGCACCGTGGGAACAGAAAAAATGA