Detailed information of TA system TA096986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3306410..3307037
• Replicon: chromosome
• Accession: NZ_CP026985
• Organism: Pectobacterium parmentieri strain IFB5619

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: C6DB72
• Locus tag: C5E25_RS15485
• Protein ID: WP_005976087.1
• Coordinates: 3306834..3307037 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: C5E25_RS15480
• Protein ID: WP_121247122.1
• Coordinates: 3306410..3306778 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E25_RS15465	3301881..3305009	+	3129	WP_015730969.1	multidrug efflux RND transporter permease subunit	-
C5E25_RS15470	3305242..3305490	+	249	WP_015730970.1	type B 50S ribosomal protein L31	-
C5E25_RS15475	3305508..3305651	+	144	WP_005976091.1	type B 50S ribosomal protein L36	-
C5E25_RS15480	3306410..3306778	+	369	WP_121247122.1	Hha toxicity modulator TomB	Antitoxin
C5E25_RS15485	3306834..3307037	+	204	WP_005976087.1	hemolysin expression modulator Hha	Toxin
C5E25_RS15495	3307765..3308085	+	321	WP_005976085.1	MGMT family protein	-
C5E25_RS15500	3308105..3308689	-	585	WP_121247125.1	YbaY family lipoprotein	-
C5E25_RS15505	3308900..3309763	+	864	WP_014700820.1	acyl-CoA thioesterase II	-
C5E25_RS15510	3309881..3311167	-	1287	WP_014700821.1	ammonium transporter AmtB	-
C5E25_RS15515	3311207..3311545	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8145.52 Da
• Isoelectric Point: 8.9008

>T96986 WP_005976087.1 NZ_CP026985:3306834-3307037 [Pectobacterium parmentieri]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Nucleotide

• Length: 204 bp

>T96986 NZ_CP026985:3306834-3307037 [Pectobacterium parmentieri]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACGATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCCAATGATGAACTGGAGATGTTCTTTTCTGCAGCCGATCATCGCCTGGCAGAGTTGACGATGAACAAAC
TCTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14004.71 Da
• Isoelectric Point: 4.3689

>AT96986 WP_121247122.1 NZ_CP026985:3306410-3306778 [Pectobacterium parmentieri]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELQRWQKSKAKLFGMFSGENVCTPAKT

Nucleotide

• Length: 369 bp

>AT96986 NZ_CP026985:3306410-3306778 [Pectobacterium parmentieri]
ATGGATGAGTACACACCAAAGCATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGATGAGAGCATAGC
AACGTTAGGCGATAGCAGTCACGGCTGGGTCAATGATCCAACTTCTGCGATCAATCTCCAATTAAATGAGCTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTCTCAGAGCAGGTTGAAAAATATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGTATTAATGACGCTGAACTACAACGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A1D7Z3F0