Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 4359060..4360096 Replicon chromosome
Accession NZ_CP026982
Organism Pectobacterium parmentieri strain IFB5486

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A8B3FNB9
Locus tag C5E22_RS20305 Protein ID WP_033070784.1
Coordinates 4359060..4359635 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A8B3FHU5
Locus tag C5E22_RS20310 Protein ID WP_033070783.1
Coordinates 4359635..4360096 (-) Length 154 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5E22_RS20285 4354881..4356143 - 1263 WP_033070786.1 integrase arm-type DNA-binding domain-containing protein -
C5E22_RS20290 4356685..4356897 + 213 WP_082218588.1 DUF1133 family protein -
C5E22_RS20295 4356959..4357783 + 825 WP_127087856.1 hypothetical protein -
C5E22_RS20300 4357770..4358891 + 1122 WP_033070785.1 XRE family transcriptional regulator -
C5E22_RS20305 4359060..4359635 - 576 WP_033070784.1 PIN domain-containing protein Toxin
C5E22_RS20310 4359635..4360096 - 462 WP_033070783.1 helix-turn-helix domain-containing protein Antitoxin
C5E22_RS20315 4360401..4360619 + 219 Protein_3898 hypothetical protein -
C5E22_RS20325 4360788..4360862 - 75 Protein_3899 type II toxin-antitoxin system RelE/ParE family toxin -
C5E22_RS23860 4361077..4361328 + 252 WP_127087855.1 hypothetical protein -
C5E22_RS20330 4361875..4362753 + 879 WP_033070781.1 integrase domain-containing protein -
C5E22_RS20335 4362756..4362956 + 201 WP_014698606.1 AlpA family transcriptional regulator -
C5E22_RS20340 4362953..4363489 + 537 WP_033070780.1 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4352170..4369176 17006


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-115)

Antitoxin

SinI-like, DNA-binding domain

(84-131)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21771.27 Da        Isoelectric Point: 4.8050

>T96967 WP_033070784.1 NZ_CP026982:c4359635-4359060 [Pectobacterium parmentieri]
MNHSPYPVVLDACVIYPNMLRDLLMEVGKSGLYQPKWTATIHDEWQRNLVENVPDLTREKLKRVEDLMNKALPDALVTGF
ENLMEGVSLPDLDDRHVVAAAIRSDSEVIVTFNLKDFPKESLAEFDIEAIHPDEFIADLLDLNQALVLQAVQRQRARLKN
PKKSVDEYFDILLKLGLPMTVKAITAYKLMI

Download         Length: 576 bp

>T96967 NZ_CP026982:c4359635-4359060 [Pectobacterium parmentieri]
ATGAATCATTCGCCTTACCCAGTCGTACTTGATGCCTGTGTCATTTATCCCAACATGCTCCGCGATCTGCTTATGGAGGT
TGGGAAATCGGGTCTGTATCAACCTAAATGGACGGCCACCATTCATGATGAATGGCAAAGAAATTTGGTTGAAAACGTAC
CGGATTTAACGCGTGAAAAATTGAAGCGCGTAGAGGATCTGATGAACAAAGCCCTACCTGATGCTCTTGTAACGGGCTTT
GAAAATCTTATGGAAGGGGTTTCACTCCCAGATCTTGACGATCGCCATGTTGTAGCTGCAGCGATAAGATCCGATTCAGA
GGTGATCGTAACCTTCAATCTTAAAGACTTTCCAAAGGAGAGCCTGGCTGAGTTTGATATTGAAGCAATTCATCCTGATG
AGTTTATCGCTGATTTGCTGGATTTAAATCAAGCCTTAGTTTTACAGGCTGTCCAAAGGCAGCGAGCCCGGCTAAAGAAC
CCCAAAAAATCAGTAGATGAATATTTCGACATACTACTGAAACTAGGCTTGCCGATGACGGTTAAGGCTATAACTGCTTA
TAAATTAATGATTTAG

Antitoxin


Download         Length: 154 a.a.        Molecular weight: 17321.80 Da        Isoelectric Point: 6.1440

>AT96967 WP_033070783.1 NZ_CP026982:c4360096-4359635 [Pectobacterium parmentieri]
MTSSILDNVSLPAQKEIEVALRGQRELATFLTTKFETQKISIHDANDEKHQIELPTSALRLLMNILGELAEGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEEGKLPHHKTGRHRRVLFADLMRYKAQRETESNEAMQELVDLSQELGFY

Download         Length: 462 bp

>AT96967 NZ_CP026982:c4360096-4359635 [Pectobacterium parmentieri]
ATGACTAGCTCAATTCTGGACAATGTAAGCCTTCCGGCTCAGAAGGAAATTGAGGTTGCGTTACGTGGACAAAGAGAACT
GGCAACATTTCTCACCACAAAGTTCGAAACGCAAAAAATATCGATTCACGATGCAAACGACGAAAAACACCAGATCGAAT
TGCCAACCTCAGCACTGAGATTGCTAATGAACATCTTGGGTGAACTCGCTGAAGGGAACGCGGTTCAAGTTGTCCCTGTG
CACGCTGAATTAACTACACAGGAGGCCGCCAATATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTTTAGAAGAAGG
AAAGCTTCCACATCATAAAACAGGCCGTCATCGCCGCGTTCTTTTTGCTGATTTGATGCGCTATAAAGCTCAGCGTGAAA
CTGAGAGCAATGAAGCAATGCAAGAACTGGTTGATTTGAGCCAGGAACTTGGTTTTTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNB9

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3FHU5

References