Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1152885..1153411 Replicon chromosome
Accession NZ_CP026982
Organism Pectobacterium parmentieri strain IFB5486

Toxin (Protein)


Gene name ccdB Uniprot ID A0A8B3FHC0
Locus tag C5E22_RS05165 Protein ID WP_033071873.1
Coordinates 1152885..1153190 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID C6C380
Locus tag C5E22_RS05170 Protein ID WP_015855076.1
Coordinates 1153193..1153411 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5E22_RS05145 1148976..1149197 - 222 Protein_986 integrase -
C5E22_RS05150 1150233..1151564 + 1332 WP_033071876.1 hypothetical protein -
C5E22_RS05155 1151566..1152144 + 579 WP_033071875.1 hypothetical protein -
C5E22_RS05160 1152242..1152779 - 538 Protein_989 exonuclease SbcC -
C5E22_RS05165 1152885..1153190 - 306 WP_033071873.1 type II toxin-antitoxin system toxin CcdB Toxin
C5E22_RS05170 1153193..1153411 - 219 WP_015855076.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C5E22_RS05175 1153470..1154213 - 744 WP_033071872.1 hypothetical protein -
C5E22_RS23720 1154355..1154525 + 171 WP_154665134.1 hypothetical protein -
C5E22_RS05185 1155117..1155458 - 342 WP_033071870.1 hypothetical protein -
C5E22_RS05190 1155644..1155838 + 195 WP_033071869.1 DUF3950 domain-containing protein -
C5E22_RS05195 1155944..1157203 - 1260 WP_069704166.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1149420..1157370 7950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11773.64 Da        Isoelectric Point: 4.4978

>T96961 WP_033071873.1 NZ_CP026982:c1153190-1152885 [Pectobacterium parmentieri]
MQFIVYQYRRASRYKMFVDVQSDIVETPKRRMVIPLIEAHNLSEKVNNTLFPLIRIDGEDYRLMTTELSSVPVEVMGEVI
ADLGDYADEIKDAINLMFWGI

Download         Length: 306 bp

>T96961 NZ_CP026982:c1153190-1152885 [Pectobacterium parmentieri]
ATGCAATTCATTGTTTATCAATACAGACGCGCCAGCCGCTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATCCCGCTTATCGAAGCACATAACCTGTCTGAGAAAGTGAATAATACGCTGTTTCCTCTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTACCTGTTGAAGTCATGGGTGAAGTGATT
GCGGATCTTGGCGACTACGCGGATGAGATCAAAGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8178.13 Da        Isoelectric Point: 7.0135

>AT96961 WP_015855076.1 NZ_CP026982:c1153411-1153193 [Pectobacterium parmentieri]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Download         Length: 219 bp

>AT96961 NZ_CP026982:c1153411-1153193 [Pectobacterium parmentieri]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTACCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
AGTGAATGACGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCGTTTGCGGATGAAAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FHC0

Antitoxin

Source ID Structure
AlphaFold DB A0A250BG46

References