Detailed information of TA system TA096959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3535785..3536581
• Replicon: chromosome
• Accession: NZ_CP026981
• Organism: Pectobacterium parmentieri strain IFB5485

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: C5E21_RS16405
• Protein ID: WP_121247144.1
• Coordinates: 3535785..3536291 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: C5E21_RS16410
• Protein ID: WP_100683471.1
• Coordinates: 3536288..3536581 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E21_RS22830	3531265..3531435	-	171	WP_012773209.1	hypothetical protein	-
C5E21_RS16385	3531576..3531917	+	342	WP_121247131.1	hypothetical protein	-
C5E21_RS16395	3532828..3533556	+	729	WP_121247138.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
C5E21_RS16400	3533997..3535469	-	1473	WP_121247141.1	hypothetical protein	-
C5E21_RS16405	3535785..3536291	-	507	WP_121247144.1	GNAT family N-acetyltransferase	Toxin
C5E21_RS16410	3536288..3536581	-	294	WP_100683471.1	DUF1778 domain-containing protein	Antitoxin
C5E21_RS16415	3537002..3538261	+	1260	WP_121247147.1	integrase arm-type DNA-binding domain-containing protein	-
C5E21_RS16430	3539165..3539386	-	222	WP_010301503.1	helix-turn-helix domain-containing protein	-
C5E21_RS16435	3539507..3539767	+	261	WP_121247153.1	helix-turn-helix domain-containing protein	-
C5E21_RS16440	3539871..3540137	+	267	WP_121247156.1	helix-turn-helix domain-containing protein	-
C5E21_RS16445	3540528..3540785	+	258	WP_121247159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3529683..3536838	7155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18176.12 Da
• Isoelectric Point: 6.4763

>T96959 WP_121247144.1 NZ_CP026981:c3536291-3535785 [Pectobacterium parmentieri]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAIATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGILVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SILLEVEI

Nucleotide

• Length: 507 bp

>T96959 NZ_CP026981:c3536291-3535785 [Pectobacterium parmentieri]
ATGATTTCCGCCCCTGAGCCACTCCACGCCGAACATGTATTATCTTCATTCTGTTGTGGCGTGGAGTCTATGGATAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGTGCATCACGGACTTTCGTTAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCTATAGCAACGAATGCCGCTCCCGGACGTTTTCGCCGCAATATGCCCGATCCTATT
CCGGTCGTGGTACTGGGTCGTTTGGCAGTAGATAAATCATTGCATGGTCAGGGTGTTGGCCGTGCTTTGGTACGGGATGC
TGGGCTAAGAGTGATTCAGGTGGCGGATACTATCGGCATCCGAGGAATATTGGTCCATGCGCTGTCTGATGAAGCGCGGG
AATTTTACCTACGGGTGGGGTTTGAGCCTTCACCGATTGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTGGGG
AGCATTTTACTTGAAGTCGAAATCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10900.50 Da
• Isoelectric Point: 10.1968

>AT96959 WP_100683471.1 NZ_CP026981:c3536581-3536288 [Pectobacterium parmentieri]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT96959 NZ_CP026981:c3536581-3536288 [Pectobacterium parmentieri]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTGCGCATCAAGCCCGCCGAACGCGATCTGATTGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTCCTTGAAGCTGCTCGCGCCGCCGCAGAGGAAGCGTTGA
TCGATCAACGTATCATCATGGCAGATCCTGATGCTTATCAGGAATTCCTTGCCCGTTTGGATCAGGCTCCTTCGCCGAAT
GCGGCGCTACGCAAAACCATGCAAACGCCCGCACCGTGGGAACAGAAAAAATGA