Detailed information of TA system TA096919

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3627564..3628221
• Replicon: chromosome
• Accession: NZ_CP026975
• Organism: Enterobacter cloacae complex sp. strain FDAARGOS_77

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: MC67_RS18305
• Protein ID: WP_045405042.1
• Coordinates: 3627811..3628221 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: V3PWU7
• Locus tag: MC67_RS18300
• Protein ID: WP_010435322.1
• Coordinates: 3627564..3627830 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC67_RS18275	3622746..3624179	-	1434	WP_045405037.1	6-phospho-beta-glucosidase BglA	-
MC67_RS18280	3624296..3625027	-	732	WP_032660101.1	MurR/RpiR family transcriptional regulator	-
MC67_RS18285	3625079..3625390	+	312	WP_023309102.1	N(4)-acetylcytidine aminohydrolase	-
MC67_RS18290	3625556..3626215	+	660	WP_023309101.1	hemolysin III family protein	-
MC67_RS18295	3626289..3627269	-	981	WP_045405040.1	tRNA-modifying protein YgfZ	-
MC67_RS18300	3627564..3627830	+	267	WP_010435322.1	FAD assembly factor SdhE	Antitoxin
MC67_RS18305	3627811..3628221	+	411	WP_045405042.1	protein YgfX	Toxin
MC67_RS18310	3628228..3628749	-	522	WP_045405044.1	flavodoxin FldB	-
MC67_RS18315	3628851..3629747	+	897	WP_023309098.1	site-specific tyrosine recombinase XerD	-
MC67_RS18320	3629776..3630489	+	714	WP_045405046.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
MC67_RS18325	3630495..3632228	+	1734	WP_045355605.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16157.12 Da
• Isoelectric Point: 10.9865

>T96919 WP_045405042.1 NZ_CP026975:3627811-3628221 [Enterobacter cloacae complex sp.]
VVLWQSDLRVSWRSQWMSLLLHGLVAAFVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNYGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T96919 NZ_CP026975:3627811-3628221 [Enterobacter cloacae complex sp.]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTACGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10555.02 Da
• Isoelectric Point: 5.1340

>AT96919 WP_010435322.1 NZ_CP026975:3627564-3627830 [Enterobacter cloacae complex sp.]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT96919 NZ_CP026975:3627564-3627830 [Enterobacter cloacae complex sp.]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTTGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: V3PWU7