Detailed information of TA system TA096918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3374870..3375386
• Replicon: chromosome
• Accession: NZ_CP026975
• Organism: Enterobacter cloacae complex sp. strain FDAARGOS_77

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MC67_RS16955
• Protein ID: WP_045402335.1
• Coordinates: 3375102..3375386 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: MC67_RS16950
• Protein ID: WP_008501400.1
• Coordinates: 3374870..3375112 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC67_RS16935	3370867..3371607	+	741	WP_045355655.1	KDGP aldolase family protein	-
MC67_RS16940	3371723..3372859	+	1137	WP_045402331.1	lactonase family protein	-
MC67_RS16945	3372882..3374792	+	1911	WP_023310188.1	BglG family transcription antiterminator	-
MC67_RS16950	3374870..3375112	+	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MC67_RS16955	3375102..3375386	+	285	WP_045402335.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MC67_RS16960	3375390..3375854	-	465	WP_045402338.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
MC67_RS16965	3376002..3378140	-	2139	WP_045402340.1	anaerobic ribonucleoside-triphosphate reductase	-
MC67_RS16970	3378517..3380160	-	1644	WP_045402342.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10907.73 Da
• Isoelectric Point: 10.3350

>T96918 WP_045402335.1 NZ_CP026975:3375102-3375386 [Enterobacter cloacae complex sp.]
MTYELAFDPRALKEWSKLGQTVKDQFKKKLADVLVNPRVESARLHGLPDCYKIKLRTQGYRLVYQVQDNVVTVFVIAIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T96918 NZ_CP026975:3375102-3375386 [Enterobacter cloacae complex sp.]
ATGACTTATGAACTGGCATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGCAGACTGTAAAAGACCAGTTTAA
GAAGAAACTTGCGGACGTGCTGGTGAACCCCAGGGTCGAATCGGCCCGCCTGCATGGTCTGCCGGATTGCTACAAAATAA
AACTCAGAACGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAACGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
CGAGAGAAATCAGCCGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT96918 WP_008501400.1 NZ_CP026975:3374870-3375112 [Enterobacter cloacae complex sp.]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT96918 NZ_CP026975:3374870-3375112 [Enterobacter cloacae complex sp.]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACGGAAGCCGTGCGATTACTGTTTCAGTACGTCGCGGAGACGGGACGTATGCCGGTGAAAACGGTCACGATTAGCGACA
GTGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCGAATCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: V3EXX3