Detailed information of TA system TA096917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2815248..2815864
• Replicon: chromosome
• Accession: NZ_CP026975
• Organism: Enterobacter cloacae complex sp. strain FDAARGOS_77

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MC67_RS14230
• Protein ID: WP_080298642.1
• Coordinates: 2815248..2815619 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: MC67_RS14235
• Protein ID: WP_023309753.1
• Coordinates: 2815622..2815864 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC67_RS14215	2812748..2813650	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
MC67_RS14220	2813647..2814282	+	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
MC67_RS14225	2814279..2815208	+	930	WP_045404233.1	formate dehydrogenase accessory protein FdhE	-
MC67_RS14230	2815248..2815619	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
MC67_RS14235	2815622..2815864	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
MC67_RS14240	2816064..2816984	+	921	WP_045404232.1	alpha/beta hydrolase	-
MC67_RS14245	2816993..2817934	-	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
MC67_RS14250	2817979..2818416	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MC67_RS14255	2818413..2819294	-	882	WP_023309756.1	virulence factor BrkB family protein	-
MC67_RS14260	2819288..2819887	-	600	WP_045404231.1	glucose-1-phosphatase	-
MC67_RS14265	2820008..2820808	-	801	WP_045404230.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T96917 WP_080298642.1 NZ_CP026975:c2815619-2815248 [Enterobacter cloacae complex sp.]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T96917 NZ_CP026975:c2815619-2815248 [Enterobacter cloacae complex sp.]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT96917 WP_023309753.1 NZ_CP026975:c2815864-2815622 [Enterobacter cloacae complex sp.]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT96917 NZ_CP026975:c2815864-2815622 [Enterobacter cloacae complex sp.]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0