Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1529208..1529828 Replicon chromosome
Accession NZ_CP026975
Organism Enterobacter cloacae complex sp. strain FDAARGOS_77

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag MC67_RS07665 Protein ID WP_008499287.1
Coordinates 1529610..1529828 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag MC67_RS07660 Protein ID WP_008499288.1
Coordinates 1529208..1529582 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC67_RS07650 1524334..1525527 + 1194 WP_023310601.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MC67_RS07655 1525550..1528696 + 3147 WP_023310600.1 multidrug efflux RND transporter permease subunit -
MC67_RS07660 1529208..1529582 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
MC67_RS07665 1529610..1529828 + 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
MC67_RS07670 1530034..1530585 + 552 WP_023310599.1 maltose O-acetyltransferase -
MC67_RS07675 1530703..1531170 + 468 WP_045400719.1 membrane protein -
MC67_RS07680 1531142..1532602 - 1461 WP_045400715.1 PLP-dependent aminotransferase family protein -
MC67_RS07685 1532704..1533414 + 711 WP_023310596.1 GNAT family N-acetyltransferase -
MC67_RS07690 1533411..1533551 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MC67_RS07695 1533554..1533814 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T96914 WP_008499287.1 NZ_CP026975:1529610-1529828 [Enterobacter cloacae complex sp.]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T96914 NZ_CP026975:1529610-1529828 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTCGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT96914 WP_008499288.1 NZ_CP026975:1529208-1529582 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT96914 NZ_CP026975:1529208-1529582 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References