Detailed information of TA system TA096885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1433741..1434270
• Replicon: chromosome
• Accession: NZ_CP026964
• Organism: Staphylococcus aureus strain FDAARGOS_2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK61_RS07255
• Protein ID: WP_000621175.1
• Coordinates: 1433908..1434270 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK61_RS07250
• Protein ID: WP_000948331.1
• Coordinates: 1433741..1433911 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK61_RS07220	1428777..1429337	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
RK61_RS07225	1429546..1430025	+	480	WP_001287078.1	hypothetical protein	-
RK61_RS07230	1430018..1431601	+	1584	WP_001294626.1	PH domain-containing protein	-
RK61_RS07235	1431588..1432079	+	492	WP_001205912.1	PH domain-containing protein	-
RK61_RS07240	1432083..1432442	+	360	WP_000581197.1	holo-ACP synthase	-
RK61_RS07245	1432508..1433656	+	1149	WP_001281154.1	alanine racemase	-
RK61_RS07250	1433741..1433911	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK61_RS07255	1433908..1434270	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK61_RS07265	1434619..1435620	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RK61_RS07270	1435739..1436065	+	327	WP_001052491.1	anti-sigma factor antagonist	-
RK61_RS07275	1436067..1436546	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
RK61_RS07280	1436521..1437291	+	771	WP_001041108.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T96885 WP_000621175.1 NZ_CP026964:1433908-1434270 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T96885 NZ_CP026964:1433908-1434270 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT96885 WP_000948331.1 NZ_CP026964:1433741-1433911 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT96885 NZ_CP026964:1433741-1433911 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1