Detailed information of TA system TA096838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1475841..1476370
• Replicon: chromosome
• Accession: NZ_CP026960
• Organism: Staphylococcus aureus strain FDAARGOS_15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK73_RS07850
• Protein ID: WP_000621175.1
• Coordinates: 1475841..1476203 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK73_RS07855
• Protein ID: WP_000948331.1
• Coordinates: 1476200..1476370 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK73_RS07825	1472831..1473601	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
RK73_RS07830	1473576..1474055	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK73_RS07835	1474057..1474383	-	327	WP_001052491.1	anti-sigma factor antagonist	-
RK73_RS07840	1474502..1475492	-	991	Protein_1454	PP2C family protein-serine/threonine phosphatase	-
RK73_RS07850	1475841..1476203	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK73_RS07855	1476200..1476370	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK73_RS07860	1476455..1477603	-	1149	WP_001281145.1	alanine racemase	-
RK73_RS07865	1477669..1478028	-	360	WP_000581200.1	holo-ACP synthase	-
RK73_RS07870	1478032..1478523	-	492	WP_001205910.1	PH domain-containing protein	-
RK73_RS07875	1478510..1480093	-	1584	WP_001294626.1	PH domain-containing protein	-
RK73_RS07880	1480086..1480565	-	480	WP_001287088.1	hypothetical protein	-
RK73_RS07885	1480774..1481334	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T96838 WP_000621175.1 NZ_CP026960:c1476203-1475841 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T96838 NZ_CP026960:c1476203-1475841 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT96838 WP_000948331.1 NZ_CP026960:c1476370-1476200 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT96838 NZ_CP026960:c1476370-1476200 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1