Detailed information of TA system TA096831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1884988..1885517
• Replicon: chromosome
• Accession: NZ_CP026958
• Organism: Staphylococcus aureus strain FDAARGOS_40

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK98_RS09910
• Protein ID: WP_000621175.1
• Coordinates: 1885155..1885517 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK98_RS09905
• Protein ID: WP_000948331.1
• Coordinates: 1884988..1885158 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK98_RS09875	1880025..1880585	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
RK98_RS09880	1880793..1881272	+	480	WP_001287087.1	hypothetical protein	-
RK98_RS09885	1881265..1882848	+	1584	WP_001294626.1	PH domain-containing protein	-
RK98_RS09890	1882835..1883326	+	492	WP_001205912.1	PH domain-containing protein	-
RK98_RS09895	1883330..1883689	+	360	WP_000581197.1	holo-ACP synthase	-
RK98_RS09900	1883755..1884903	+	1149	WP_001281154.1	alanine racemase	-
RK98_RS09905	1884988..1885158	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK98_RS09910	1885155..1885517	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK98_RS09920	1885866..1886867	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RK98_RS09925	1886986..1887312	+	327	WP_001052491.1	anti-sigma factor antagonist	-
RK98_RS09930	1887314..1887793	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK98_RS09935	1887768..1888538	+	771	WP_001041106.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T96831 WP_000621175.1 NZ_CP026958:1885155-1885517 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T96831 NZ_CP026958:1885155-1885517 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT96831 WP_000948331.1 NZ_CP026958:1884988-1885158 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT96831 NZ_CP026958:1884988-1885158 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1