Detailed information of TA system TA096814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2805054..2805583
• Replicon: chromosome
• Accession: NZ_CP026957
• Organism: Staphylococcus aureus strain FDAARGOS_43

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RL01_RS14540
• Protein ID: WP_000621175.1
• Coordinates: 2805221..2805583 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RL01_RS14535
• Protein ID: WP_000948331.1
• Coordinates: 2805054..2805224 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL01_RS14505	2800090..2800650	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
RL01_RS14510	2800859..2801338	+	480	WP_001287078.1	hypothetical protein	-
RL01_RS14515	2801331..2802914	+	1584	WP_001294626.1	PH domain-containing protein	-
RL01_RS14520	2802901..2803392	+	492	WP_001205912.1	PH domain-containing protein	-
RL01_RS14525	2803396..2803755	+	360	WP_000581197.1	holo-ACP synthase	-
RL01_RS14530	2803821..2804969	+	1149	WP_001281154.1	alanine racemase	-
RL01_RS14535	2805054..2805224	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RL01_RS14540	2805221..2805583	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RL01_RS14550	2805931..2806932	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RL01_RS14555	2807051..2807377	+	327	WP_001052491.1	anti-sigma factor antagonist	-
RL01_RS14560	2807379..2807858	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
RL01_RS14565	2807833..2808603	+	771	WP_001041108.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T96814 WP_000621175.1 NZ_CP026957:2805221-2805583 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T96814 NZ_CP026957:2805221-2805583 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT96814 WP_000948331.1 NZ_CP026957:2805054-2805224 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT96814 NZ_CP026957:2805054-2805224 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1