Detailed information of TA system TA096780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 596644..597173
• Replicon: chromosome
• Accession: NZ_CP026953
• Organism: Staphylococcus aureus strain FDAARGOS_48

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RL06_RS03375
• Protein ID: WP_000621175.1
• Coordinates: 596644..597006 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RL06_RS03380
• Protein ID: WP_000948331.1
• Coordinates: 597003..597173 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL06_RS03350	593622..594392	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
RL06_RS03355	594367..594846	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
RL06_RS03360	594848..595174	-	327	WP_001052491.1	anti-sigma factor antagonist	-
RL06_RS03365	595293..596294	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RL06_RS03375	596644..597006	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RL06_RS03380	597003..597173	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RL06_RS03385	597258..598406	-	1149	WP_001281154.1	alanine racemase	-
RL06_RS03390	598472..598831	-	360	WP_000581197.1	holo-ACP synthase	-
RL06_RS03395	598835..599326	-	492	WP_001205912.1	PH domain-containing protein	-
RL06_RS03400	599313..600896	-	1584	WP_001294626.1	PH domain-containing protein	-
RL06_RS03405	600889..601368	-	480	WP_001287087.1	hypothetical protein	-
RL06_RS03410	601576..602136	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T96780 WP_000621175.1 NZ_CP026953:c597006-596644 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T96780 NZ_CP026953:c597006-596644 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT96780 WP_000948331.1 NZ_CP026953:c597173-597003 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT96780 NZ_CP026953:c597173-597003 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1