Detailed information of TA system TA096765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 39008..39384
• Replicon: plasmid pCFS3313-1
• Accession: NZ_CP026940
• Organism: Escherichia coli strain CFS3313

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C5F74_RS23710
• Protein ID: WP_001514250.1
• Coordinates: 39008..39190 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: C5F74_RS23715
• Protein ID: WP_001324224.1
• Coordinates: 39187..39384 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F74_RS23700	37007..38041	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
C5F74_RS23705	38601..38909	-	309	WP_000377483.1	hypothetical protein	-
C5F74_RS23710	39008..39190	-	183	WP_001514250.1	hypothetical protein	Toxin
C5F74_RS23715	39187..39384	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
C5F74_RS23720	40099..41340	+	1242	WP_001332356.1	HlyD family secretion protein	-
C5F74_RS23725	41315..43429	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
C5F74_RS23730	43599..43910	-	312	WP_001259758.1	colicin V	-
C5F74_RS23735	43888..44124	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..155172	155172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-100)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6961.10 Da
• Isoelectric Point: 4.5349

>T96765 WP_001514250.1 NZ_CP026940:c39190-39008 [Escherichia coli]
MMEIFWTMLTSQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T96765 NZ_CP026940:c39190-39008 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGACCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT96765 WP_001324224.1 NZ_CP026940:c39384-39187 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT96765 NZ_CP026940:c39384-39187 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA