96758

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3649730..3650348 Replicon chromosome
Accession NZ_CP026939
Organism Escherichia coli strain CFS3313

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5F74_RS17630 Protein ID WP_001291435.1
Coordinates 3650130..3650348 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5F74_RS17625 Protein ID WP_000344800.1
Coordinates 3649730..3650104 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F74_RS17615 3644819..3646012 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C5F74_RS17620 3646035..3649184 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C5F74_RS17625 3649730..3650104 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5F74_RS17630 3650130..3650348 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C5F74_RS17635 3650520..3651071 + 552 WP_000102568.1 maltose O-acetyltransferase -
C5F74_RS17640 3651187..3651657 + 471 WP_000136192.1 YlaC family protein -
C5F74_RS17645 3651821..3653371 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5F74_RS17650 3653413..3653766 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C5F74_RS17660 3654067..3654456 + 390 WP_001300427.1 DNA base-flipping protein -
C5F74_RS17665 3654487..3655059 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96758 WP_001291435.1 NZ_CP026939:3650130-3650348 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96758 NZ_CP026939:3650130-3650348 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96758 WP_000344800.1 NZ_CP026939:3649730-3650104 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96758 NZ_CP026939:3649730-3650104 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References