Detailed information of TA system TA096756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2560008..2560646
• Replicon: chromosome
• Accession: NZ_CP026939
• Organism: Escherichia coli strain CFS3313

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C5F74_RS12220
• Protein ID: WP_000813794.1
• Coordinates: 2560470..2560646 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C5F74_RS12215
• Protein ID: WP_001270286.1
• Coordinates: 2560008..2560424 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F74_RS12190	2555325..2555978	-	654	Protein_2386	ABC transporter ATP-binding protein	-
C5F74_RS12195	2555994..2556691	-	698	Protein_2387	IS1 family transposase	-
C5F74_RS12200	2556747..2557115	-	369	Protein_2388	ABC transporter ATP-binding protein	-
C5F74_RS12205	2557133..2558278	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
C5F74_RS12210	2558523..2559929	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
C5F74_RS12215	2560008..2560424	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C5F74_RS12220	2560470..2560646	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C5F74_RS12225	2560868..2561098	+	231	WP_000494244.1	YncJ family protein	-
C5F74_RS12230	2561190..2563151	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C5F74_RS12235	2563224..2563760	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
C5F74_RS12240	2563813..2565027	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2565067..2566332	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T96756 WP_000813794.1 NZ_CP026939:c2560646-2560470 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T96756 NZ_CP026939:c2560646-2560470 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT96756 WP_001270286.1 NZ_CP026939:c2560424-2560008 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT96756 NZ_CP026939:c2560424-2560008 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB