Detailed information of TA system TA096745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1414824..1415449
• Replicon: chromosome
• Accession: NZ_CP026939
• Organism: Escherichia coli strain CFS3313

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C5F74_RS06805
• Protein ID: WP_000911330.1
• Coordinates: 1415051..1415449 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C5F74_RS06800
• Protein ID: WP_000450524.1
• Coordinates: 1414824..1415051 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F74_RS06775	1410627..1411097	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C5F74_RS06780	1411097..1411669	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C5F74_RS06785	1411815..1412693	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C5F74_RS06790	1412710..1413744	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C5F74_RS06795	1413957..1414670	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C5F74_RS06800	1414824..1415051	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C5F74_RS06805	1415051..1415449	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C5F74_RS06810	1415596..1416459	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C5F74_RS06815	1416474..1418489	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
C5F74_RS06820	1418563..1419261	+	699	WP_000679823.1	esterase	-
C5F74_RS06825	1419371..1419571	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T96745 WP_000911330.1 NZ_CP026939:1415051-1415449 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T96745 NZ_CP026939:1415051-1415449 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT96745 WP_000450524.1 NZ_CP026939:1414824-1415051 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT96745 NZ_CP026939:1414824-1415051 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33