Detailed information of TA system TA096744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1125121..1125704
• Replicon: chromosome
• Accession: NZ_CP026939
• Organism: Escherichia coli strain CFS3313

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C5F74_RS05405
• Protein ID: WP_021548855.1
• Coordinates: 1125369..1125704 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: C5F74_RS05400
• Protein ID: WP_000581937.1
• Coordinates: 1125121..1125369 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F74_RS05390	1121460..1122761	+	1302	WP_000046790.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
C5F74_RS05395	1122809..1125043	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
C5F74_RS05400	1125121..1125369	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C5F74_RS05405	1125369..1125704	+	336	WP_021548855.1	endoribonuclease MazF	Toxin
C5F74_RS05410	1125775..1126566	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
C5F74_RS05415	1126794..1128431	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
C5F74_RS05420	1128519..1129817	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
C5F74_RS05425	1129873..1130235	-	363	WP_000034929.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12186.13 Da
• Isoelectric Point: 8.4777

>T96744 WP_021548855.1 NZ_CP026939:1125369-1125704 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPVVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T96744 NZ_CP026939:1125369-1125704 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGTTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT96744 WP_000581937.1 NZ_CP026939:1125121-1125369 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT96744 NZ_CP026939:1125121-1125369 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LMB4