Detailed information of TA system TA096742

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 844948..845783
• Replicon: chromosome
• Accession: NZ_CP026939
• Organism: Escherichia coli strain CFS3313

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: C5F74_RS04095
• Protein ID: WP_001094445.1
• Coordinates: 844948..845325 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C5F74_RS04100
• Protein ID: WP_024174331.1
• Coordinates: 845415..845783 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F74_RS04065	840356..841333	+	978	WP_000633239.1	type II secretion system minor pseudopilin GspK	-
C5F74_RS04070	841330..842508	+	1179	WP_000094989.1	type II secretion system protein GspL	-
C5F74_RS04075	842510..843046	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
C5F74_RS04080	843327..844169	-	843	WP_001280499.1	DUF4942 domain-containing protein	-
C5F74_RS04085	844254..844451	-	198	WP_000838536.1	DUF957 domain-containing protein	-
C5F74_RS04090	844463..844951	-	489	WP_000761717.1	hypothetical protein	-
C5F74_RS04095	844948..845325	-	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
C5F74_RS04100	845415..845783	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5F74_RS04105	845857..846078	-	222	WP_000692346.1	DUF987 domain-containing protein	-
C5F74_RS04110	846147..846623	-	477	WP_001384029.1	RadC family protein	-
C5F74_RS04115	846639..847124	-	486	WP_021538211.1	antirestriction protein	-
C5F74_RS04120	847216..848034	-	819	WP_021548586.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T96742 WP_001094445.1 NZ_CP026939:c845325-844948 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T96742 NZ_CP026939:c845325-844948 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT96742 WP_024174331.1 NZ_CP026939:c845783-845415 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT96742 NZ_CP026939:c845783-845415 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6