Detailed information of TA system TA096739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 52905..53506
• Replicon: plasmid pCFS3292-3
• Accession: NZ_CP026938
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: doc
• Uniprot ID: F4TND7
• Locus tag: C5F72_RS27155
• Protein ID: WP_001216030.1
• Coordinates: 52905..53285 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: C5F72_RS27160
• Protein ID: WP_072649184.1
• Coordinates: 53285..53506 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS27130	48346..49830	-	1485	WP_032163774.1	hypothetical protein	-
C5F72_RS27135	49830..51023	-	1194	WP_156838212.1	terminase	-
C5F72_RS27140	51109..51561	-	453	WP_016231381.1	hypothetical protein	-
C5F72_RS27145	51650..52693	-	1044	WP_047649471.1	DUF968 domain-containing protein	-
C5F72_RS27150	52721..52900	-	180	WP_000113018.1	hypothetical protein	-
C5F72_RS27155	52905..53285	-	381	WP_001216030.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C5F72_RS27160	53285..53506	-	222	WP_072649184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C5F72_RS27165	53689..55245	+	1557	WP_038997084.1	type I restriction-modification system subunit M	-
C5F72_RS27170	55242..56435	+	1194	WP_159195488.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99789	99789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13601.29 Da
• Isoelectric Point: 5.1408

>T96739 WP_001216030.1 NZ_CP026938:c53285-52905 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T96739 NZ_CP026938:c53285-52905 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGATATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8105.04 Da
• Isoelectric Point: 4.7708

>AT96739 WP_072649184.1 NZ_CP026938:c53506-53285 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELANR

Nucleotide

• Length: 222 bp

>AT96739 NZ_CP026938:c53506-53285 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGCTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A1M1W8Q3