Detailed information of TA system TA096738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 70844..71379
• Replicon: plasmid pCFS3292-2
• Accession: NZ_CP026937
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C5F72_RS26600
• Protein ID: WP_001270421.1
• Coordinates: 71092..71379 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: C5F72_RS26595
• Protein ID: WP_001132895.1
• Coordinates: 70844..71095 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS26555	66465..66656	-	192	WP_032200969.1	hypothetical protein	-
C5F72_RS26560	66653..66931	-	279	WP_166509434.1	DNA methylase	-
C5F72_RS26565	66900..67106	-	207	WP_166513035.1	hypothetical protein	-
C5F72_RS26570	67106..67408	-	303	WP_001666994.1	antirestriction protein	-
C5F72_RS26575	67605..67778	+	174	Protein_81	DUF4113 domain-containing protein	-
C5F72_RS26580	67919..68881	-	963	WP_032200967.1	ParB/RepB/Spo0J family partition protein	-
C5F72_RS26585	68878..70083	-	1206	WP_000817642.1	AAA family ATPase	-
C5F72_RS26590	70458..70670	+	213	WP_000402944.1	hypothetical protein	-
C5F72_RS26595	70844..71095	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C5F72_RS26600	71092..71379	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5F72_RS26605	71381..71791	+	411	Protein_87	DDE-type integrase/transposase/recombinase	-
C5F72_RS26610	72435..73412	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
C5F72_RS26615	73691..74431	-	741	WP_001066941.1	tyrosine-type recombinase/integrase	-
C5F72_RS26620	74552..74719	-	168	WP_162841962.1	transcriptional regulator	-
C5F72_RS26625	75171..75868	-	698	Protein_91	IS1 family transposase	-
C5F72_RS26630	75925..76071	-	147	Protein_92	ester cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia / aac(3)-IIa / blaTEM-1A	-	1..131954	131954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T96738 WP_001270421.1 NZ_CP026937:71092-71379 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T96738 NZ_CP026937:71092-71379 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCTTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCAGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT96738 WP_001132895.1 NZ_CP026937:70844-71095 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT96738 NZ_CP026937:70844-71095 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAAATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTGGACTTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6