Detailed information of TA system TA096731

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4652701..4653536
• Replicon: chromosome
• Accession: NZ_CP026935
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F2VI91
• Locus tag: C5F72_RS22860
• Protein ID: WP_000854748.1
• Coordinates: 4653159..4653536 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0IVG5
• Locus tag: C5F72_RS22855
• Protein ID: WP_001339471.1
• Coordinates: 4652701..4653069 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS22825	4649516..4649971	+	456	WP_000581504.1	hypothetical protein	-
C5F72_RS22830	4650050..4650283	+	234	WP_001119729.1	DUF905 family protein	-
C5F72_RS22835	4650383..4651201	+	819	WP_001234631.1	DUF945 domain-containing protein	-
C5F72_RS22840	4651283..4651762	+	480	WP_159376051.1	antirestriction protein	-
C5F72_RS22845	4651778..4652254	+	477	WP_001186727.1	RadC family protein	-
C5F72_RS22850	4652317..4652538	+	222	WP_000692345.1	DUF987 domain-containing protein	-
C5F72_RS22855	4652701..4653069	+	369	WP_001339471.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5F72_RS22860	4653159..4653536	+	378	WP_000854748.1	TA system toxin CbtA family protein	Toxin
C5F72_RS22865	4653533..4654021	+	489	WP_000761721.1	hypothetical protein	-
C5F72_RS22870	4654033..4654230	+	198	WP_000839281.1	DUF957 domain-containing protein	-
C5F72_RS22875	4654327..4654899	+	573	WP_001290246.1	DUF4942 domain-containing protein	-
C5F72_RS22880	4655659..4656887	+	1229	WP_167745260.1	IS3 family transposase	-
C5F72_RS22885	4656984..4658522	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4629892..4666334	36442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14179.16 Da
• Isoelectric Point: 7.7761

>T96731 WP_000854748.1 NZ_CP026935:4653159-4653536 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T96731 NZ_CP026935:4653159-4653536 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13517.22 Da
• Isoelectric Point: 6.4789

>AT96731 WP_001339471.1 NZ_CP026935:4652701-4653069 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT96731 NZ_CP026935:4652701-4653069 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: F2VI91

Antitoxin

• AlphaFold DB: A0A0H0IVG5