Detailed information of TA system TA096730

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4576010..4576507
• Replicon: chromosome
• Accession: NZ_CP026935
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: C5F72_RS22505
• Protein ID: WP_001173343.1
• Coordinates: 4576334..4576507 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: C5F72_RS22500
• Protein ID: WP_096936272.1
• Coordinates: 4576010..4576306 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS22470	4571639..4571878	-	240	Protein_4402	transposase	-
C5F72_RS22475	4571882..4572862	-	981	WP_159376047.1	IS110 family transposase	-
C5F72_RS22480	4573167..4574192	+	1026	WP_097707938.1	HoxN/HupN/NixA family nickel/cobalt transporter	-
C5F72_RS22485	4574266..4574852	+	587	Protein_4405	YcxB family protein	-
C5F72_RS22490	4575257..4575430	-	174	Protein_4406	transposase domain-containing protein	-
C5F72_RS22495	4575682..4575966	+	285	WP_096848713.1	hypothetical protein	-
C5F72_RS22500	4576010..4576306	+	297	WP_096936272.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
C5F72_RS22505	4576334..4576507	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
C5F72_RS22510	4576626..4578143	-	1518	WP_137427006.1	lysine--tRNA ligase	-
C5F72_RS22515	4578458..4580605	-	2148	WP_137426808.1	lysine decarboxylase CadA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	f17d-G / f17d-C / f17d-D / f17d-A	4516464..4595666	79202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (6-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T96730 WP_001173343.1 NZ_CP026935:4576334-4576507 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T96730 NZ_CP026935:4576334-4576507 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11463.08 Da
• Isoelectric Point: 4.3808

>AT96730 WP_096936272.1 NZ_CP026935:4576010-4576306 [Escherichia coli]
MKCKNRFNTYVVSFDYPSSYSSVFLRLRSLMCDMNFSSIVADEYGIPRQLNENSLAITTSLSASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT96730 NZ_CP026935:4576010-4576306 [Escherichia coli]
ATGAAATGTAAAAATAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTGTGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTGGCAATCACAACATCACTCTCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41